This helped in conceptualization of an ‘ in sitro’ ( in vitro+ in situ) sFIS assay, where human myeloid cells were exposed to patients’ serum in vitro, to assess serum-induced (si)-NFκB or interferon (IFN)/ISG responses (as active signaling reporter activity) within them, thereby ‘mimicking’ patients’ in situ immunodynamic status. Results In silico data-driven framework predicted that peripheral immunodynamics of patients with cancer might be best captured via analyzing myeloid nuclear factor kappa-light-chain enhancer of activated B cells (NFκB) signaling and interferon-stimulated genes' (ISG) responses. Correspondence to Professor Abhishek D Garg.16 Laboratory for Molecular Digestive Oncology, Department of Oncology, KU Leuven, Leuven, Belgium.15 VIB Center for Cancer Biology, VIB, Leuven, Belgium.14 Department of Cellular and Molecular Medicine, Cell Death Research and Therapy Laboratory, KU Leuven, Belgium.13 Department of Microbiology, Immunology and Transplantation, Laboratory of Virology and Chemotherapy, Rega Institute, KU Leuven, Leuven, Belgium.12 Department of Gynaecology and Obstetrics, UZ Leuven, Leuven, Belgium.11 National Cancer Institute of Milan, Milan, Italy.10 Ziekenhuis Oost-Limburg, Genk, Belgium.9 Charles University, Praha, Czech Republic.8 Dipartimento Scienze della Vita e Sanità pubblica, Università Cattolica del Sacro Cuore, Rome, Italy.Gemelli, Istituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy 7 Dipartimento Scienze della Salute della Donna e del Bambino, Fondazione Policlinico Universitario A.6 Queen Charlotte's and Chelsea Hospital, Imperial College, London, UK.5 Department of Development and Regeneration, KU Leuven, Leuven, Belgium.3 Department of Oncology, Leuven Cancer Institute, Laboratory of Gynaecologic Oncology, KU Leuven, Leuven, Belgium.2 Department of Oncology, Leuven Cancer Institute, Laboratory of Tumor Immunology and Immunotherapy, KU Leuven, Leuven, Belgium.
1 Laboratory of Cell Stress & Immunity, Department of Cellular & Molecular Medicine, KU Leuven, Leuven, Belgium.
0 kommentar(er)
